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The Cowboy Junkies are an alternative country and folk rock band formed in Toronto, Ontario, Canada in 1985 by Alan Anton (bassist), Michael Timmins (songwriter, guitarist), Peter Timmins (drummer) and Margo Timmins (vocalist). The three Timminses are siblings, and Anton worked with Michael Timmins during their first couple of bands. John Timmins was initially a member of the band but left the group before the recording of their first album. The band line-up has never changed since, although they use several guest musicians on many of their albums, including multi-instrumentalist Jeff Bird who has performed on every album except the first.

The Cowboy Junkies' 1986 debut album, produced by Canadian producer Peter Moore, was the blues-inspired Whites Off Earth Now!!, recorded in the family garage using a single ambisonic microphone.

The band gained wide recognition with their second album, The Trinity Session, recorded in 1987 at Toronto's Church of the Holy Trinity. Their sound, again using the ambisonic microphone, and their mix of blues, country, folk, rock and jazz earned them both critical attention and a strong fan base. The Los Angeles Times named the recording one of the 10 best albums of 1988.

Their fame in the United States grew after the single "Sweet Jane" was used in the film and on the soundtrack for the 1994 film Natural Born Killers.

The Cowboy Junkies have gone on to record 16 studio albums and five live albums, and remain active as of 2020.